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Protein & Peptide Letters

Author(s): Woo Young Kang and Young Kee Chae

DOI: 10.2174/092986610790780378

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Stationary Phase Expression, Purification, and Characterization of XorKI, a Restriction Endonuclease from Xanthomonas oryzae pv. oryzae

Page: [381 - 385] Pages: 5

  • * (Excluding Mailing and Handling)

Abstract

An endonuclease from Xanthomonas oryzae pathovar oryzae KACC 10331, XorKI, was heterologously produced in Escherichia coli by applying the stationary state induction method. The yield was 5.4 mg of XorKI per liter of LB medium. XorKI existed in multiple oligomeric forms as evidenced by gel filtration chromatography. The specific activity of purified XorKI was 323000 units per mg.

Keywords: XorKI, restriction endonuclease, Xanthomonas, stationary state induction