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Protein & Peptide Letters

Author(s): Shinya Kida, Yusuke Eto, Yasuo Yoshioka, Shinsaku Nakagawa, Koichi Kawasaki and Mitsuko Maeda

DOI: 10.2174/092986610790225978

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Evaluation of Synthetic Cell-Penetrating Peptides, Pro-Rich Peptide and Octaargine Derivatives, as Adenovirus Vector Carrier

Page: [164 - 167] Pages: 4

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Abstract

Two cell-penetrating peptides, a Pro-rich peptide derivative, acetyl-(Val-Arg-Leu-Pro-Pro-Pro)3-Gly-Cys amide, and an octaarginine derivative, acetyl-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Cys amide, were prepared by the solid phase method. Each peptide was coupled to the heterobifunctional cross-linking reagent, 6-maleimidohexanoic acid N-hydroxysuccinimide ester, and then conjugated to the Adenovirus vector containing luciferase gene. Peptide-modified Ad, as compared with wild-type Ad, exhibited excellent luciferase activity in B16BL6 cells.

Keywords: Adenovirus, Pro-rich peptide, octaarginine, adenovirus vector, peptide synthesis, cell-penetrating peptide