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Protein & Peptide Letters

Author(s): Alessia Ruggiero, Flavia Squeglia, Carla Esposito, Daniela Marasco, Emilia Pedone, Carlo Pedone and Rita Berisio

DOI: 10.2174/092986610789909557

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Expression, Purification, Crystallization and Preliminary X-Ray Crystallographic Analysis of the Resuscitation Promoting Factor Interacting Protein RipA from M. tuberculosis

Page: [70 - 73] Pages: 4

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Abstract

RipA is an important growth factor of M. tuberculosis. Its depletion produces decreasing bacterial growth and abnormal phenotype. RipA C-terminal fragment (263-472), containing its predicted catalytic domain has been successfully crystallized using vapor-diffusion methods. The structure has been solved by Multiwavelength Anomalous Dispersion and atomic resolution refinement is in progress.

Keywords: Resuscitation promoting factor, crystal, growth factor, x-ray, tuberculosis