Author(s): Sadaf Fatima and Rizwan Hasan Khan
Page: [423 - 429] Pages: 7
* (Excluding Mailing and Handling)
The equilibrium denaturation pathway of Succinyl Con A exhibited three-state mechanism with the transition midpoints at 1 and 3 M GdnHCl and at 2.6 and 5 M urea. Unfolding resulted in stabilization of molten-globule (MG) like intermediate states at 2 M GdnHCl and 3 M urea. It was particularly interesting that state obtained at 3 M urea was functionally active.
Keywords: Succinylated Con A, Molten Globule like state, equilibrium denaturation, guanidine hydrochloride, urea
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