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Protein & Peptide Letters

Author(s): Keigo Hisamatsu, Hideaki Unno, Shuichiro Goda and Tomomitsu Hatakeyama

DOI: 10.2174/092986609787848054

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Roles of the Valine Clusters in Domain 3 of the Hemolytic Lectin CEL-III in Its Oligomerization and Hemolytic Abilities

Page: [411 - 414] Pages: 4

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Abstract

The hemolytic lectin CEL-III and its site-directed mutants were expressed in Escherichia coli cells. Replacement of the valine clusters in domain 3 with alanine residues led to increased self-oligomerization in solution and higher hemolytic activity. The results suggest the involvement of these valine clusters in CEL-III oligomerization and hemolytic activity.

Keywords: Cucumaria echinata, hemolysin, lectin, oligomerization, site-directed mutagenesis