Protein & Peptide Letters

Author(s): Beatriz Dos Santos Ferreira, Alan Trindade Branco, Karlla Salim de Queiroz and Goncalo Apolinario de Souza Filho

DOI: 10.2174/0929866023409057

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Two-dimensional Protein Analysis by Isoelectric Focusing in a Multi-cell Plate System

Page: [39 - 43] Pages: 5

  • * (Excluding Mailing and Handling)

Abstract

A practical and low cost system for isoelectric protein focusing (IEF) was developed. The system uses a multi-cell glass plate compatible with a common vertical one-dimensional electrophoresis chamber, dispensing specific IEF apparatus. After focusing, the IEF gels are easily recovered. The resulting two-dimensional (2-D) gels have provided efficient protein separation for different concentrations and extracts.

Keywords: isoelectric protein focusing (IEF), sodium dodecylsulfate (SDS)-PAGE, mercaptoethanol, Ampholytes solution, maize endosperm