Protein & Peptide Letters

Author(s): A. Morana, A. Esposito, L. Maurelli, G. Ruggiero, E. Ionata, M. Rossi and F. La Cara

DOI: 10.2174/092986608785849209

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A Novel Thermoacidophilic Cellulase from Alicyclobacillus acidocaldarius

Page: [1017 - 1021] Pages: 5

  • * (Excluding Mailing and Handling)

Abstract

A novel cellulase was isolated from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius ATCC27009 grown in medium containing carboxymethylcellulose. The enzyme is a glycosylated monomer of 56.2 kDa, relatively thermostable, with optimal pH and temperature of 4.0 and 65 °C, respectively. Enzymatic assays on several polysaccharides demonstrated that CelG was specific for carboxymethylcellulose.

Keywords: Alicyclobacillus acidocaldarius, carboxymethylcellulose, cellulase, glycoprotein, cellooligosaccharides