Protein & Peptide Letters

Author(s): Cesare Giordano and Sergio Ammendola

DOI: 10.2174/092986608784966949

Characterization of Mutants of Sulfolobus solfataricus Signature Amidase Able to Hydrolyse R-Ketoprofen Amide

Page: [617 - 623] Pages: 7

  • * (Excluding Mailing and Handling)

Abstract

The amidase from Sulfolobus solfataricus enantioselectively hydrolyzes S-ketoprofen amide to its corresponding acid. We identify three independent SsAH mutants that hydrolyze R-ketoprofen amide and built computational models of their three-dimensional structure. Interestingly the mutations do not specifically affect residues near the active site, or directly interacting with the substrate.

Keywords: Enantioselectivity, Amidase, Mutation, Modelling, R-Ketoprofen