Abstract

During analysis of the proteome in the cerebrospinal fluid (CSF) of the Caucasian form of moyamoya disease (MMD), a novel post-translational modification of human transthyretin was observed. Two-dimensional electrophoresis and subsequent peptide sequencing with ESI-MS/MS were performed to discover the γ-carboxylation of the Glu-42 (Gla- 42).

Keywords: γ-Carboxy-glutamate, cerebrospinal fluid, moyamoya disease, post-translational modification, human transthyretin, two-dimensional gel electrophoresis