This work reportes the effects of isolated proteins from algaroba (Prosopis juliflo ra D.C.) seeds fractionated by ammonium sulfate at saturation 0-30percent., 30-60percent and 60-90percent. They were tested, in vitro, against papain and midgut homogenate of C. maculatus larvae. Also, the effects in vivo these protein fractions on the development of C. maculatus larvae were tested. F 30 / 60 fraction was effective against papain (72percent inhibition), midgut homogenate of larvae of C. maculatus (59,2percent inhibition) and in vivo with WD50 of 1,7percent
Keywords: Callosobruchus maculatus, Prosopis juliflora, proteinases