Abstract

A new antifungal protein, named as GAFP-1, was purified from leaves of Ginkgo biloba L. Its Molecular weight identified by mass spectrometry is 25988.0 Da and pI is 4.3. The analysis of amino acids composition showed it has high percentages of Gly and Glu / Gln. The 15 N-terminal amino acid residues of GAFP-1 were determined. GAFP-1 could inhibit the hyphal growth of P. sasakii Ito, the IC50 towards P.sasakii Ito is 250micro gram / ml. GAFP-1 has chitinase activity, the optimal pH and temperature being 5.0 and 50 C.

Keywords: ANTIFUNGAL PROTEIN, acetylglucosamine, GAFP-1