Author(s): Chen Li. Wei, Feng Ya. Ping, Zhou De He, Xu xue-Jun, Chen Jie, Wei Qiang and Chi Zhi-Qiang
Page: [265 - 272] Pages: 8
* (Excluding Mailing and Handling)
Affinity chromatography on Ni-nitrilotriacetic acid agarose was used to purify human mu-opioid receptors overexpressed in Sf9 cells. We introduced a tag of 6 consecutive histidines at its carboxyl terminus for easy purification of recombinant proteins. The binding activity and identification of the purified receptor were determined by receptor binding assay, SDS-PAGE and Western blotting analysis. This procedure used in the paper offer an easy and fast route to the purification of recombinant human mu-opioid receptors.
Keywords: BACULOVIRUS, opioid receptor (MOR), Spodoptera frugiperda, Trichoplusia ni, Receptor binding assay
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