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Protein & Peptide Letters

Author(s): Quan-Sheng Liu, Jian-Hua Liu, De-Sheng Jiang, You-Xin Jin and De-Bao Wang

DOI: 10.2174/0929866013409265

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Isolation and Identification of Two Membrane Proteins Binding to Transfer RNA

Page: [367 - 374] Pages: 8

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Abstract

Two major tRNA binding proteins were obtained by biochemical method from yeast crude extracts of total membrane proteins. They were further confirmed to be tRNA specific-binding proteins by gel mobility shift assay. N-terminal sequence assays showed that they were glyceraldehyde-3-phosphate dehydranase (GAPDH) and 3-phosphate glycerate kinase (PGK1), respectively.

Keywords: glyceraldehyde-3-phosphate dehydranase (GAPDH), RNA polymerase III