Generic placeholder image

Protein & Peptide Letters

Author(s): Paresh L. Gadhavi, Steven Howell, Anthony I. Magee and Roger S. Buxton

DOI: 10.2174/0929866013409247

DownloadDownload PDF Flyer Cite As
Nucleolin Binds to a cis-Active Region in the Human Desmocollin 2 Promoter

Page: [349 - 356] Pages: 8

  • * (Excluding Mailing and Handling)

Explore Articles
About Journal
For Authors
For Editors
For Reviewers

Abstract

Affinity chromatography using oligo DNA from a cis-active region (-453 / -389) of the human DSC2 promoter was used to isolate a protein from human CaCo-2 epithelial cells expressing DSC2. The protein was SDS-PAGE purified, trypsinized and analyzed by MALDI-TOF mass spectrometry. Peptide mass fingerprinting identified the protein as nucleolin, a ubiquitously expressed protein involved in gene regulation

Keywords: NUCLEOLIN, DESMOCOLLIN 2 PROMOTER, Glycoproteins, desmosomal cadherins desmocollins (DSC), desmogleins (DSG), plakoglobin (PG), plakophilin (PKP), desmoplakin (DP)