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Protein & Peptide Letters

Author(s): Zdzislaw Wawrazak, Paul V. Viitanen, Joseph C. Calabrese and Douglas B. Jordan

DOI: 10.2174/0929866013409698

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Crystallization And Preliminary X-Ray Diffraction Study Of Riboflavin Synthase

Page: [69 - 73] Pages: 5

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Abstract

Escherichia coli riboflavin synthase crystallizes at 22 C in the presence of 7-10percent by volume diglyme, 20-50 mM MgCl2 and pH 7.0. In this medium diffraction quality crystals are routinely obtained within 5 h and they are stable for 10 weeks. The crystals are orthogonal in space group P212121 with unit cell dimensions of a=52.4 A , b = 62.1 A, c = 218.8 A. A 97percent complete data set was collected at 2.1 A resolution.

Keywords: RIBOFLAVIN SYNTHASE