Author(s): C. S. Nagano, F.B. M.B. Moreno, C. Bloch Jr, M. V. Prates, J. J. Calvete, S. Saker-Sampaio, W.R. L. Farias, T. D. Tavares, K. S. Nascimento, T. B. Grangeiro, B. S. Cavada and A. H. Sampaio
Page: [159 - 165] Pages: 7
* (Excluding Mailing and Handling)
A lectin from the red marine alga Hypnea musciformis (HML) was purified by extraction with 20 mM PBS, precipitation with 70% saturated ammonium sulphate, ion-exchange DEAE-Cellulose chromatography and RP-HPLC. The 9.3 kDa polypeptide agglutinates erythrocytes from various sources and shows oligomerization tendencies under certain MALDI-TOF / MS conditions. Preliminary N-terminal sequencing and biological assays strongly suggest that the HML may belong to a new class of algae lectins.
Keywords: hypnea musciformis, red marine alga, lectin
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