Abstract

A synthetic modified gene encoding the human calcitonin analog (hmCT) was expressed by use of the baculovirus expression system. After injection with recombinant baculoviruses, the hmCT-GST fusion protein was produced within the silkworm larvae. The fusion protein was purified by affinity chromatography. Biological activity of hmCT for hypercalcemic effect was determined in normal rats.

Keywords: Human Calcitonin, hmCT-GST fusion, recombinant baculoviruses, silkworm larvae, hypercalcemic effect