Author(s): Venugopalan Nagarajan, Nobuyuki Sakurai, Miho Kubota, Takamasa Nonaka, Hiroaki Nagumo, Hisashi Takeda, Tomoko Nishizaki, Eiji Masai, Masao Fukuda and the late Yukio Mitsui
Page: [412 - 417] Pages: 6
* (Excluding Mailing and Handling)
The terminal oxygenase component of the biphenyl dioxygenase (BphA1A2 complex) was over-expressed with a novel over expression system in recombinant Rhodococcus strain and purified. The purified enzyme has been crystallized by the hanging drop vapor diffusion method and subjected to X-ray diffraction analysis. The crystals belong to the tetragonal system in the space group P4 1 2 1 2 or P4 3 2 1 2 and diffract to better than 2.2Å resolution.
Keywords: biphenyl dioxygenase, polychlorinated biphenyl, rieske iron-sulfur center, non-heme iron
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