Abstract

The biological potential of RNA along with the variety of possible applications has led to an increased demand for large-scale production of RNA molecules. Recent developments in the field allow for the synthesis of long RNA molecules (up to 100 nts) of excellent quality. This review focuses on current strategies (i.e. 2-O-TBDMS, 2-O-TOM and 2-O-ACE chemistry) for chemical RNA synthesis.

Keywords: oligonucleotides, phosphoramidite, protecting groups, rna, solid phase, synthesis