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Protein & Peptide Letters

Author(s): Yuichiro Kezuka, Keiji Kitazaki, Yoshikane Itoh, Jun Watanabe, Osamu Takaha, Takeshi Watanabe, Yoko Nishizawa and Takamasa Nonaka

DOI: 10.2174/0929866043406968

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Crystallization and Preliminary X-ray Analysis of Plant Class I Chitinase from Rice

Page: [401 - 405] Pages: 5

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Abstract

We report here on crystallization and preliminary X-ray analysis of plant class I chitinase from rice (OsChia1b). Similar single crystals of full-length OsChia1b were obtained under two independent conditions. The crystals grown under these conditions diffracted up to 2.1 and 2.5 Å resolution, respectively, at a synchrotron beamline, and were found to belong to the tetragonal space group P43212.

Keywords: class I chitinase, rice (oryza sativa l. japonica), crystallization, preliminary x-ray analysis, protease inhibitor complex, specificity, elastase