Protein & Peptide Letters

Author(s): Nobutada Tanaka, Masayuki Nakanishi, Yoshio Kusakabe, Yoshikuni Goto, Yukio Kitade and Kazuo T. Nakamura

DOI: 10.2174/0929866053765734

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Crystallization of the N-Terminal Ankyrin Repeat Domain of the 2-5ADependent Endoribonuclease, RNase L

Page: [387 - 389] Pages: 3

  • * (Excluding Mailing and Handling)

Abstract

The N-terminal ankyrin repeat domain of the 2-5-linked oligoadenylate (2-5A)-dependent endoribonuclease, RNase L, has been crystallized by the hanging-drop vapor diffusion method in the presence of 2-5A. The crystals belong to an orthorhombic space group P212121 with cell dimensions of a = 63.11 Å, b = 73.03 Å, and c = 82.64 Å. There is one molecule per asymmetric unit. The crystals diffract to at least 2.1 Åresolution using synchrotron radiation and are suitable for X-ray structure analysis at high resolution.

Keywords: ankyrin repeat, a system, interferon, ribonuclease l