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Protein & Peptide Letters

Author(s): Zhikang Qian, Baoqin Xuan, Jie Hong, Zhaojin Hao, Lu Wang and Weida Huang

DOI: 10.2174/0929866053765590

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Expression and Purification of the Carboxyl Terminus Domain of Schizosaccharomyces pombe Dicer in Escherichia coli

Page: [311 - 314] Pages: 4

  • * (Excluding Mailing and Handling)

Abstract

The carboxyl terminus domain of Schizosaccharomyces pombe dicer (yDicerC) was expressed in Escherichia coli as an MBP-fusion protein (MBP-yDicerC). When the E. coli strain was cultured and induced at 25°C, the MBPyDicerC was partly expressed in the soluble fraction. It was then purified by two step affinity chromatography with amylose resin and Ni-NTA His.Bind® resin. The purified MBP-yDicerC showed double-strand RNA digestion activity. siRNA-like products about 22-nt in length were generated.

Keywords: schizosaccharomyces pombe, dicer, mbp, rnai