Protein & Peptide Letters

Author(s): Xiao-Jing He, Liu Li, Hai Hou, Yaqin Zhao, Aihua Liang and Zihe Rao

DOI: 10.2174/0929866054395842

Preliminary X-Ray Crystallographic Analysis of Centrin from Ciliate Euplotes octocarinatus

Page: [609 - 611] Pages: 3

  • * (Excluding Mailing and Handling)

Abstract

Centrins are four-EF-hand Ca2+-binding proteins, which belong to the CaM super family. The centrin from ciliate Euplotes Octocarinatus has been expressed in Escherichia coli, purified and crystallized using the hanging-drop method. Rod-like crystals were grown and diffracted to 2.0 Å. The crystals belong to space group P222 and the unit-cell parameters are a=34.442 Å, b=48.954 Å, c=72.583 Å.

Keywords: centrin, crystallization, preliminary x-ray studies