Protein & Peptide Letters

Author(s): Qiang Xu, Xiaolan Ding, Yandao Gong, Tingyun Kuang and Nanming Zhao

DOI: 10.2174/0929866054395815

DownloadDownload PDF Flyer Cite As
Global Changes of Chlorophyll Excitonic Interactions in Photosystem II During Thermal Denaturation

Page: [573 - 578] Pages: 6

  • * (Excluding Mailing and Handling)

Abstract

Photosystem II (PSII) is a multisubunit chlorophyll-binding enzyme that absorbs light to catalyze water oxidation and plastoquinone reduction. Chlorophyll excitonic interaction changes in PSII were studied by absorption and circular dichroism spectra from 25°C to 80°C, and protein subunit denaturation was monitored by differential scanning calorimetry. A four-stage process of chlorophyll excitonic interaction change was observed being correlated with the denaturation of protein subunits.

Keywords: photosystem II, thermal denaturation, chlorophyll excitonic interaction, circular dichroism, absorption spectrum, differential scanning calorimetry