Abstract

Specific enzyme immobilization has moved into the focus for many applications in biochemical research fields. Expressed Protein Ligation (EPL) has been proven to be ideal to selectively label proteins at single positions. Applying this technique to enzymes of the aldo/keto reductase superfamily provides a new approach to generate native or modified redox enzymes for direct and indirect immobilization.

Keywords: expressed protein ligation (epl), site specific covalent immobilization, akr a, artificial redoxproteins, aldo/keto reductase (akr)