Abstract

Here we report a bi-directional and interchangeable three-segment peptide ligation of N, M, and C-segments, mimicking the reverse process of protein splicing to form, in tandem, a tripartite NMC-peptide using a synthetic intein, a role served by the M-segment with an N-terminal Ser or Thr and a C-terminal thioester.

Keywords: reverse protein splicing, three-segment tandem ligation, cys ligation, pro ligation, synthetic intein, c-to-n tandem ligation, n-to-c tandem ligation, tripartite nmc-protein