Author(s): Xiaoping Wu, Xiaokun Li, Zhijian Su, Qing Zheng, Hua Xu, Sixian Wu, Ya Feng and Wen Zhao
Page: [659 - 661] Pages: 3
* (Excluding Mailing and Handling)
Vasostatin has previously been expressed in fused form or in inclusion body form in Escherichia coli. Here the protein was expressed in soluble non-fusion form in BL21(DE3)pLysS by IPTG induction. The expression level of vasostatin was about 15% of the total cellular protein. The expressed vasostatin was purified and its biological activity was investigated by an endothelial cell proliferation assay.
Keywords: vasostatin, soluble expression, purification
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