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Protein & Peptide Letters

Author(s): M.L. Martin, E. Espinosa Vidal and L. de la Canal

DOI: 10.2174/092986607781483697

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Expression of a Lipid Transfer Protein in Escherichia coli and Its Phosphorylation by a Membrane-Bound Calcium-Dependent Protein Kinase

Page: [793 - 799] Pages: 7

  • * (Excluding Mailing and Handling)

Abstract

Ha-AP10 is a basic antifungal peptide from sunflower seeds (Helianthus annuus antifungal peptide of 10 kDa) belonging to the family of plant lipid transfer proteins. We report here its expression in E. coli [Glutathione S-transferase (GST) system] and its phosphorylation by endogenous membrane-bound calcium-dependent protein kinases.

Keywords: Calcium-dependent protein phosphorylation, GST expression system, Helianthus annuus, lipid transfer protein