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Protein & Peptide Letters

Author(s): Tengjiao Cui, Suzanne Joynt, Victor Morillo, Maria Baez, Zhichun Hua, Xiaotang Wang and Fenfei Leng

DOI: 10.2174/092986607779117281

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Large Scale Preparation of the Mammalian High Mobility Group Protein A2 for Biophysical Studies

Page: [87 - 91] Pages: 5

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Abstract

Due to asymmetrical charge distribution of the mammalian high mobility group protein A2 (HMGA2), which makes HMGA2 bind to both cation- and anion-exchange columns, we developed a rapid procedure for purifying HMGA2 in the milligram range. This purification procedure greatly facilitated biophysical studies, which require large amounts of the protein.

Keywords: The mammalian high mobility group protein A2 (HMGA2), DNA binding protein, isothermal titration calorimetry, differential scanning calorimetry, nuclear magnetic resonance (NMR) Spectroscopy, intrinsically unstructured protein