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Protein & Peptide Letters

Author(s): Feng Yu, Jiaping Song, Chunyan Xu, Yu Ding, Xiaojian Hu, Jianhua He and Zhihong Zhang

DOI: 10.2174/092986606777145724

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Crystallization and Preliminary X-Ray Analysis of Sau3AI C-Terminal 232-419 Amino Acids Fragment

Page: [627 - 628] Pages: 2

  • * (Excluding Mailing and Handling)

Abstract

The C-terminal 232-419 amino acids fragment of endonuclease Sau3AI has been successfully expressed in Escherichia coli with 6 His at its N-terminal. After purification and crystallization, one completed 2.8 Å data set was collected using a Rigaku R-AXIS IV ++ diffractometer. The plate-like crystals belong to orthorhombic space group P212121 with the cell dimension of a = 34.75, b = 76.82, c = 123.59Å and contain one molecule per asymmetric unit.

Keywords: Sau3AI endonuclease, crystallization, preliminary X-ray analysis