Abstract

Purified malate dehydrogenase (MDH) of Streptomyces aureofaciens was crystallized either in the absence or in the presence of NADH or NADPH coenzymes by hanging-drop vapour-diffusion method. An X-ray study has shown, that MDH crystals belong to space group C2221 with unit-cell parameters A = 53.2 Å, b = 104.6 Å, c = 520.0 Å, α = β = γ = 90° , MDH-NADH crystals to space group C2 with unit-cell parameters A = 51.5 Å, b = 51.5 Å, c = 256 Å, α = β = γ = 90 , and MDH-NADPH crystals to space group C2221 with unit-cell parameters A = 72, Å b = 72 Å, c = 520 Å, α = β = γ = 90° . The crystal of native MDH diffracted to 2.1 Å resolution.

Keywords: Malate dehydrogenase (MDH), Streptomyces aureofaciens, protein crystallization