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Protein & Peptide Letters

Author(s): Yusuke Kato, Yoriko Sawano and Masaru Tanokura

DOI: 10.2174/092986606775101670

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Expression and Purification of Active WW Domains of FBP11/HYPA and FBP28/CA150

Page: [197 - 201] Pages: 5

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Abstract

Production of GST-fused WW domains of FBP proteins was increased using the bubbling cultivation method for E. coli. Purified WW domains of FBP11 and FBP28 bound A PL motif peptide with dissociation constants (KD) of 248 ± 27 and 1880 ± 280 μM, respectively.

Keywords: WW domain, air pump, reverse phase chromatography, surface plasmon resonance