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Protein & Peptide Letters

Author(s): Lie Wang, Dan Xu, Ming Qian, Guanzhen Yang and Xiangfu Wu

DOI: 10.2174/092986606775101553

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Identification and Characterization of Trichoplusia ni Furin Truncated Mutant

Page: [171 - 175] Pages: 5

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Abstract

We report A new prohormone convertase gene of insect origin, Trichoplusia ni furin, which was cloned from BTI-Tn-5B-4 (Tn5) insect cells. We constructed A truncated mutant that lacked Cys-rich repeated segments. Using baculovirus expression system and standard enzymatic assay, we obtained recombinant Tn furin and evaluated aspects of its function.

Keywords: Trichoplusia ni furin, baculovirus, Cys-rich repeated segment, Tn -5B1-4 insect cells