Abstract

It is now widely accepted that oxidative stress induced by reactive oxygen and nitrogen species is involved in the pathogenesis of various diseases such as atherosclerosis and cardiovascular disease and consequently the role of antioxidants in the prevention and treatment of diseases has received much attention of scientists, clinicians and general public. However, most of the large clinical intervention trials of antioxidants and meta-analysis of the data from these large studies do not show beneficial evidence with regard to cardiovascular outcomes. In order to understand the role of antioxidants, it is essential to elucidate the action and capacity of antioxidants. In this article, the assessment of antioxidant capacity is reviewed and the methods for assessment of natural antioxidant capacity are discussed.

Keywords: Antioxidant, antioxidant capacity, atherosclerosis, lipid peroxidation, low density lipoprotein, vitamin E, oxidative stress, cardiovascular disease, antioxidants, meta-analysis, reactive oxygen/nitrogen species (ROS/RNS), density lipoprotein (LDL), untracentrifugation, phosphatidylcholine (PC), lipophilic core, enzymatic oxidation, Linoleates, polyunsaturated fatty acid (PUFA), arachidonates, Carotenoids, Coenzyme Q, 12Ehydropero-xyoctadecadienoic acid (HPODE), prostaglandin (PG), isoprostanes (isoPs), cyclooxygenase, Aracidonic, biomarker, oxysterols, hydroxycholesterols, Superoxide dismutase (SOD), lipophilic antioxidants, spectrophotometer, 2,2-diphenyl-1-picrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC), total radical antioxidant parameter (TRAP), Trolox equivalent antioxidant capacity (TEAC), total oxyradical scavenging capacity (TOSC), liposomal membranes, low density lipoprotein (LDL), plasma oxidation, lipoproteins, Phosphatidylcholine, vitamin C, uric acid, cholesteryl linoleate hydroxide, Bioavailability, erythrocytes, cerebrospinal fluids, Neuroprostane, Neurofuran, Dityrosine, Myeloperoxidase, myelopreroxidase (MPO), high density lipoprotein (HDL), Area under curve, Hydroxyeicosatetraenoic acid, 4-Hydroxy-2-nonenal, Hydroperoxyeicosatetraenoic acid, Hydoxyoctadecadienoic acid