Abstract

Deoxyribonucleic acid (DNA) biosensor based on a carbon nanotube (CNT) modified electrode was developed as an anticancer drug screening device for rapid electrochemical detection of cyclophosphamide. Screen-printing technology was employed to fabricate electrodes for biosensor construction. The interaction of cyclophosphamide with double stranded (ds) calf thymus DNA was detected with the developed CNT-based biosensor and compared with the carbon paste based biosensor by using differential pulse voltammetry. The preliminary results indicate that the developed CNT-based DNA biosensor exhibited a faster response and a higher detection reproducibility compared to the carbon paste based biosensor.

Keywords: Anti-cancer drug, electrochemical DNA biosensors, cyclophosphamide, carbon nanotubes, anticancer drug interactions