Abstract

Background: This study developed a UV spectrophotometric method for quantifying allopurinol and lycopene in both bulk and dosage forms, aiming for simplicity and practicality.

Materials and Methods: The method was developed and validated using methanol and lyco-pene in phosphate buffer saline at various pH levels (7.4, 6.8, 1.2, 5.5). Validation parameters included linearity, accuracy, precision, limit of quantification (LOQ), and limit of detection (LOD) according to ICH standards.

Results: The method demonstrated high sensitivity and linearity over a concentration range of 4-20 μg/ml, adhering to Beer's law. The LOQ and LOD were 2–18 μg/ml and 2–6 μg/ml, re-spectively. Optimal absorbance wavelengths were 251 nm for lycopene and 471 nm for allopu-rinol. Calibration curves showed a high correlation coefficient (0.9994), indicating a strong lin-ear relationship.

Conclusion: The developed UV spectrophotometric method is effective, interference-free, and suitable for routine quality control, providing reliable measurements for allopurinol and lyco-pene.

Keywords: Bioanalysis, allopurinol, lycopene, spectroscopy, precise, calibration