Abstract

Background: Microorganisms associated with sea sponges have proven to be good natural product resources that are biologically active and pharmaceutically important.

Objective: This research aimed to identify actinomycetes related to a sponge from Barranglompo Island Makassar and the antibacterial compounds.

Methods: Identification of actinomycetes was based on molecular characterization of sequence gen16S rRNA. The antibacterial compound was separated using vacuum liquid chromatography and preparative Thin Layer Chromatography (TLC). The structure determination was done based on spectroscopy 1H-NMR, 13C-NMR, 2D NMR, and mass spectra.

Results: Molecular characterization showed that actinomycetes strain BLP 20 had the closest relationship with Streptomyces parvulus and Uncultured Streptomyces sp. with a similarity value of 83%. The results obtained from the characterization of antibacterial compounds based on spectroscopic data indicate that these compounds lead to Actinomycin D.

Conclusion: Characterization and identification of Strain 20 / BLP by molecular phylogenetic analysis of 16S rRNA sequences revealed the closest relationship with Uncultured Streptomyces sp and S. parvulus with a similarity value of 83%, which indicated a new species. The structure of the active compound isolated from actinomycetes strain 20 / BLP leads to Actinomycin D.

Keywords: Sponge, streptomyces parvulus, actinomycin D, 16S rRNA gene, antibacterial, barrang lompo island.