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Anti-Infective Agents

Author(s): Somayeh Dehghani Sanij, Pegah Shakib* and Abbas Morovvati*

DOI: 10.2174/0122113525314697240711070000

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The Frequency of Aminoglycoside and Vancomycin-resistant Genes in Enterococci Isolated from Chicken Meat in Qom City, Iran

Article ID: e22113525314697 Pages: 7

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Abstract

Background and Purpose: Considering the emergence of antibiotic resistance in enterococci and the possibility of transmission of resistant strains to humans, their spread in natural reservoirs is of particular importance. The food chain is known as a source of enterococci that is resistant to antimicrobial agents, and these bacteria are present in foods of animal origin, such as meat and milk. Hence, the purpose of this study was to investigate the frequency of aminoglycoside- and vancomycin-resistant genes in Enterococcus spp isolates isolated from chicken meat in Qom City, Iran.

Methods: After collecting and culturing 200 chicken meat samples from September 2021 to August 2022, enterococci were isolated and identified using biochemical and microbial standard tests. Antibiotic sensitivity and resistance of the isolates, aph(2")1c ، aph(2")1b ، aph(2")1d ، ant(3') ، aph(3')IIIa ، ant(4')1a ،ant( و (' 6 aac(6'), vanA and vanB genes were determined.

Results: From 200 chicken meat samples, 40(40%) isolates of Enterococcus faecalis and 60 (60%) isolates of Enterococcus faecium were isolated. Resistance to gentamicin, tobramycin, amikacin, neomycin, tetracycline, chloramphenicol, vancomycin, and ampicillin were 33, 30, 33, 20, 69, 38, 28, and 45, respectively. Frequency of aph(2")1c, aph(2")1b, aph(2")1d, ant(3'), aph(3')IIIa, ant(4')1a, ant(6') and aac (6'), vanA and vanB were reported to be 13%, 2%, 3%, 45%, 20%, 7%, 19%, 21%, 7%, and 6%, respectively.

Conclusion: To reduce the infection with this organism and to prevent and control its spread and mortality, investigating the epidemiology and determining the frequency of resistance to aminoglycosides are important.

Keywords: Enterococcus, aminoglycoside, vancomycin, antimicrobial resistance, chicken meat, cell wall.

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