Generic placeholder image

Infectious Disorders - Drug Targets

Author(s): Vaishnavi Vishram Naik, Pooja Rao, Ethel Suman and Udayalaxmi Jeppu*

DOI: 10.2174/0118715265301138240605071106

DownloadDownload PDF Flyer Cite As
Antibacterial effect of Aloe Vera on Bacteria Isolated from Cases of Wound Infection

Article ID: e280624231439

  • * (Excluding Mailing and Handling)

Explore Articles
About Journal
For Authors
For Editors
For Reviewers

Abstract

Background: Antimicrobial resistance exhibited by bacteria against the major-ity of antibiotics has resulted in research on alternative methods of treatment. Aloe vera has a strong tradition as a medical plant with a wide range of therapeutic uses.

Objective: The objective of this study is to determine the antibacterial activity of gel and crude ethanol leaf extract of Aloe vera against Staphylococcus aureus and Enterobacter-ales isolated from wound infections.

Methods: It is a cross-sectional study conducted over a period of 7 months. Antibacterial effect of the ethanol leaf extract and gel was determined by the punch well method. Min-imum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the ethanol leaf extract were determined by macro broth dilution technique.

Results: Aloe vera ethanol leaf extract induced a mean zone size of 13.0 ± 6.0 mm and 16.7 ± 8.4 mm, respectively, for S. aureus and Enterobacterales by Punch Well method (p≤0.002). Whereas Aloe vera gel failed to induce any zone of inhibition for all the isolates p<0.001. Mean MIC of Aloe vera leaf extract against 74 S. aureus was 94 ± 41.23 mg/ml and against 73 Enterobacterales, it was 45.6 ± 20 mg/ml p < 0.001. Mean MBC of Aloe vera leaf extract against 74 S.aureus isolates was 188 ± 82.46 mg/ml and against 73 En-terobacterales was 91.18±40 mg/ml p < 0.001.

Conclusion: Aloe vera ethanol leaf extract showed a good antibacterial effect against the different strains of bacteria causing wound infection. The present article shows the possi-bility of future use of natural products for the treatment of wound infections.

[1]
Mohammed A, Seid ME, Gebrecherkos T, Tiruneh M, Moges F. Bacterial isolates and their antimicrobial susceptibility patterns of wound infections among inpatients and outpatients attending the university of Gondar Referral Hospital, Northwest Ethiopia. Int J Microbiol 2017; 2017: 1-10.
[http://dx.doi.org/10.1155/2017/8953829] [PMID: 28386280]
[2]
Roopashree S, Prathab AG. T S. Bacteriological profile and antibiotic susceptibility patterns of wound infections in a tertiary care hospital in South India. Indian J Microbiol Res 2021; 8(1): 76-85.
[http://dx.doi.org/10.18231/j.ijmr.2021.016]
[3]
Saleem A, Naureen I, Naeem M, Murad HS, Maqsood S, Tasleem G. Aloe vera gel effect on skin and pharmacological properties. Schol. Int J Anat Physiol 2022; 5(1): 1-8.
[http://dx.doi.org/10.36348/sijap.2022.v05i01.001]
[4]
Amalia N, Fajrina A, Eriadi A. Antimicrobial activity test of aloe vera plant extract: A review article. Sci Int 2020; 32(6): 721-7.
[5]
Danish P, Ali Q, Hafeez MM, Malik A. Antifungal and antibacterial activity of aloe vera plant extract. Biolog Clin Sci Res J 2020; 2020: e004.
[http://dx.doi.org/10.54112/bcsrj.v2020i1.4]
[6]
Hekmatpou D, Mehrabi F, Rahzani K, Aminiyan A. The effect of Aloe vera clinical trials on prevention and healing of skin wound: A systematic review. Iran J Med Sci 2019; 44(1): 1-9.
[PMID: 30666070]
[7]
Prasad SD, Goda PC, Reddy KS, Kumar CS, Hemadri M, Ranga Reddy DS. Evaluation of antimicrobial efficacy of neem and Aloe vera leaf extracts in comparison with 3% sodium hypochlorite and 2% chlorhexidine against E. faecalis and C. albicans. J NTR Univ Health Sci 2016; 5(2): 104-10.
[http://dx.doi.org/10.4103/2277-8632.185436]
[8]
Liu C, Cui Y, Pi F, Cheng Y, Guo Y, Qian H. Extraction, purification, structural characteristics, biological activities and pharmacological applications of acemannan, a polysaccharide from Aloe vera: A review. Molecules 2019; 24(8): 1554.
[http://dx.doi.org/10.3390/molecules24081554] [PMID: 31010204]
[9]
Adzitey F, Agbolosu AA, Udoka UJ. Antibacterial Effect of Aloe Vera Gel Extract on Escherichia coli and Salmonella enterica Isolated from the Gastrointestinal Tract of Guinea Fowls. World Vet J 2019; 9(3): 166-73.
[http://dx.doi.org/10.36380/scil.2019.wvj21]
[10]
Arbab S, Ullah H, Weiwei W, et al. Comparative study of antimicrobial action of aloe vera and antibiotics against different bacterial isolates from skin infection. Vet Med Sci 2021; 7(5): 2061-7.
[http://dx.doi.org/10.1002/vms3.488] [PMID: 33949142]
[11]
Crichton PB. Enterobacteriaceae: Escherichia, klebsiella, proteus and other genera. In: Collee JG, Fraser GA, Marmion BP, Simmons A, Eds. Mackie & McCartney Practical Medical Microbiology. New York: Churchill Livingstone, Elsevier publishers 2012; pp. 361-404.
[12]
Goudarzi M, Fazeli M, Azad M, Seyedjavadi SS, Mousavi R. Aloe vera gel: Effective therapeutic agent against multidrug resistant Pseudomonas aeruginosa isolates recovered from burn wound infections. Chemother Res Pract 2015; 2015: 1-5.
[http://dx.doi.org/10.1155/2015/639806] [PMID: 26266047]
[13]
Nortjie E, Basitere M, Moyo D, Nyamukamba P. Extraction Methods, Quantitative and Qualitative Phytochemical Screening of Medicinal Plants for Antimicrobial Textiles: A Review. Plants 2022; 11(15): 2011.
[http://dx.doi.org/10.3390/plants11152011] [PMID: 35956489]
[14]
Hayat KM, Kumar T, Ahmad N, Jan SA, Sajjad W, Faisal S. In vitro antimicrobial activity of Aloe vera L. extracts against pathogenic bacteria and fungi. Mycopath 2016; 14: 21-7.
[15]
Waithaka PN, Eliud MG, Benson MG, Kazungu R Z. Antimicrobial properties of Aloe vera, Aloe volkensii and Aloe secundiflora from Egerton University. Acta Sci Microbiol 2018; 1(5): 06-10.
[16]
Rudrangshu C, Dushyant S, Dimri AG, Pandita A, Chaudhary S, Aggarwal ML. Comparative study of antimicrobial activity of Aloe vera gel and antibiotics against isolates. World J Pharm Pharm Sci 2015; 4(4): 1054-8.
[17]
Arsene MMJ, Viktorovna PI, Sergei GV, et al. Phytochemical analysis, antibacterial and antibiofilm activities of Aloe vera aqueous extract against selected resistant gram-negative bacteria involved in urinary tract infections. Fermentation 2022; 8(11): 626.
[http://dx.doi.org/10.3390/fermentation8110626]
[18]
Cataldi V, Bartolomeo DS, Campli DE, Nostro A, Cellini L, Di Giulio M. In vitro activity of Aloe vera inner gel against microorganisms grown in planktonic and sessile phases. Int J Immunopathol Pharmacol 2015; 28(4): 595-602.
[http://dx.doi.org/10.1177/0394632015600594] [PMID: 26526205]
[19]
Jain S, Rathod N, Nagi R, et al. Antibacterial effect of Aloe vera gel against oral pathogens: An in-vitro study. J Clin Diagn Res 2016; 10(11): ZC41-4.
[http://dx.doi.org/10.7860/JCDR/2016/21450.8890] [PMID: 28050502]
[20]
Darshan D, Pagi N, Jasani H, Patel P. Antimicrobial Activity and Phytochemical Screening of Aloe vera (Aloe barbadensis Miller). Int J Curr Microbiol Appl Sci 2017; 6: 2152-62.
[http://dx.doi.org/10.20546/ijcmas.2017.603.246]
[21]
Azzahra S, Parisa N. Antibacterial efficacy of Aloe vera sap against Staphylococcus aureus and Escherichia coli. J Biomed Transl Res 2019; 3(2): 29-37.
[22]
Yahya R, Al-Rajhi AMH, Alzaid SZ, et al. Molecular docking and efficacy of Aloe vera gel based on chitosan nanoparticles against Helicobacter pylori and its antioxidant and anti-inflammatory activities. Polymers 2022; 14(15): 2994.
[http://dx.doi.org/10.3390/polym14152994] [PMID: 35893958]
[23]
Joseph AMM, Jorelle AB, Sarra S, Viktorovna PI, Davares AK, Ingrid NK. Short review on the potential alternatives to antibiotics in the era of antibiotic resistance. J Appl Pharm Sci 2022; 12: 29-40.