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Journal of Current Toxicology and Venomics

Author(s): Mhaveer Singh*, Kamal Y.T., Mohammad A. Khan, Navneet Verma, Arun K. Mishra and Sayeed Ahmad

DOI: 10.2174/0126661217302439240513160410

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Elucidation of the Possible Molecular Mechanism for the Anti-Apoptotic Effect of Terpene-Rich Extract of Amomum subulatum and Coriandrum sativum against Rodent Model of Cardiotoxicity

Article ID: e050624230706 Pages: 13

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Abstract

Background: Spices, including Amomum subulatum and Coriander sativum, are not only popular for improving food flavour but are also accepted worldwide for their health benefits in various disorders.

Objective: This study aimed to elucidate the possible mechanism for the anti-apoptotic effect of terpene-rich extract of Amomum subulatum and Coriandrum sativum against the rodent model of cardiotoxicity.

Methods: Effects of standardized terpenoids-rich aqueous methanolic extract of two commonly used spices, viz. Amomum subulatum Roxb. and Coriandrum sativum Linn., were investigated on doxorubicin-induced apoptotic changes and cardiotoxicity in Wistar rats with the aim of investigating the mechanism. Prior to the in vivo experiment, the extracts were subjected to quantitative estimation of possible bioactive markers of the terpenes by employing a newly developed, optimized, and validated GC-MS method along with TLC profiling.

Results: Cardiotoxicity was evident from elevated creatinine kinase (CK-MB), lactate dehydrogenase (LDH), and malondialdehyde (MDA) levels in the toxic control group after treatment with doxorubicin (2.5 mg/kg i.p. given twice a week for three weeks). Caspase-3, Tumor necrosis factor-α (TNF-α), and Interleukin-6 (IL-6) were also induced in animals treated with doxorubicin. Treatment with Amomum subulatum and Coriandrum sativum at the doses of 100 and 200 mg/kg exhibited significant (P<0.001) reversal of CK-MB, LDH, MDA, Caspase-3, TNF-α, and IL-6 levels. This protective effect was further supported by the results of DNA gel electrophoresis and histopathological observations.

Conclusion: This study supports the cardioprotective role of selected spices against doxorubicininduced cardiotoxicity through the anti-apoptotic mechanism.

Keywords: Amomum subulatum Roxb., Coriandrum sativum Linn., doxorubicin, cardiovascular disease, apoptosis, caspase-3.

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