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Current Pharmaceutical Design

Author(s): Mahnaz Mohtashami, Alieh Rezagholizade-Shirvan, Zahra Hojati Bonab, Mohammad Reza Amiryousefi*, Majid Darroudi, Mobina Sadat Ahmadi Solimani, Sajad Yaghoobi, Samaneh Dolatabadi, Ahmad Ghasemi and Amir Abbas Momtazi-Borojeni*

DOI: 10.2174/0113816128304460240408085736

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Green Synthesis of Silver Nanoparticles using Cirsium congestum Extract Modified by Chitosan/Alginate: Bactericidal Activity against Pathogenic Bacteria and Cytotoxicity Analysis in Normal Cell Line

Page: [1610 - 1623] Pages: 14

  • * (Excluding Mailing and Handling)

Abstract

Aim: The study aimed to determine in vitro pharmacological effects of modified Ag nanoparticles (AgNPs).

Background: AgNPs are considered antimicrobial agents. However, the cytotoxicity of chemically synthesized AgNPs (cAgNPs) has raised challenges that limit their use.

Objective: The purpose of the study was to examine the antimicrobial and cytotoxicity effects of AgNPs synthesized using Cirsium congestum extract modified by chitosan/alginate AgNPS (Ch/ALG-gAgNPs).

Methods: Nanoparticles were characterized using TEM, DLS, XRD, and FTIR. Resistant strains of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were used for the antimicrobial analysis of Ch/ALG-gAgNPs using disc diffusion and microdilution methods. The effects of NPs on cell viability and apoptosis in L929 normal cells were determined using MTT assay and annexin/PI staining, respectively.

Results: Physicochemical characterizations confirmed Ch/ALG-gAgNPs to be spherical and uniformly dispersed, and their size ranged from 50 to 500 nm. Ch/ALG-gAgNPs inhibited the growth of microbial strains in a dose-dependent manner. The antibacterial effect of Ch/ALG-gAgNPs was significantly higher than cAgNPs. The Ch/ALG-gAgNPs showed little cytotoxicity against normal cells at concentrations less than 50 μg/ml. Cytotoxicity effects of Ch/ALG-gAgNP were less than cAgNPs. Flow cytometry and real-time PCR results showed a decrease in apoptosis percentage and BAX marker in the presence of Ch/ALG-gAgNPs relative to when the cell was treated with cAgNPs.

Conclusion: Current findings introduce novel gAgNPs modified with chitosan/alginate for use in medicine.

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