Generic placeholder image

Current Nanomedicine

Author(s): A. Krishna Sailaja* and Aisha Tabassum

DOI: 10.2174/0124681873283442240228054238

DownloadDownload PDF Flyer Cite As
Formulation of Letrozole-loaded Ethyl Cellulose and Eudragit S100 Nanoparticles by Nanoprecipitation Technique and Determination of Cytotoxic Activity by MTT Assay

  • * (Excluding Mailing and Handling)

Explore Articles
About Journal
For Authors
For Editors
For Reviewers

Abstract

Introduction: The major goal of this work is to develop letrozole nanoparticles using the polymer precipitation technique. Formulations were prepared by using Ethyl cellulose and Eudragit S100 as polymers.

Methods: By varying drug-polymer ratios, a total of ten formulations were prepared. By altering the drug concentration to polymer, five formulations were prepared with Ethyl cellulose and five with Eudragit S100. All ten formulations were evaluated for different characterization and evaluation parameters such as Entrapment efficiency, Loading capacity and in vitro drug release studies, particle size, stability (zeta potential), surface morphology, and drug-polymer interaction study.

Result: In comparison, the NEC 2:1 formulation showed the smallest particle size,high stability, good entrapment efficiency, and sustained drug release. This formulation was further studied to determine the anticancer activity in vitro in the MCF-7 Breast cancer cell line by MTT assay. The results indicated that the prepared formulation exhibited anticancer activity with an IC50 value of 91.26 micromolar.

Conclusion: Comparatively, Ethyl cellulose was proven to be a better polymer than Eudragit S100, and the nanoprecipitation technique was considered the most suitable technique for preparing letrozole nanoparticles.

[1]
Naik JB, Lokhande AB. Development of sustained release micro/nanoparticles using different solvent emulsification techniques: A review. Int J Pharm Bio Sci 2012; 3(4): 573-90.
[2]
Avanço GB, Bruschi ML. Preparation and characterisation of ethylcellulose microparticles containing propolis. Rev Cienc Farm Basica Apl 2008; 29(2): 129-35.
[3]
Gulbake A, Jain SK. Colon specific delivery of mesalazine using biocompatible polymeric nanoparticles. Int J Pharm Pharm Technol 2013; 1(2): 1994-2175.
[4]
Gao W, Chan J, Omid C. pH-Responsive nanoparticles for drug delivery. Mol Pharm 2010; 7(6): 1913-020.
[5]
Hu D, Liu L, Chen W, Li S, Zhao Y. A novel preparation method for 5-aminosalicylic acid loaded Eudragit S100 nanoparticles. Int J Mol Sci 2012; 13(5): 6454-68.
[http://dx.doi.org/10.3390/ijms13056454] [PMID: 22754377]
[6]
Costa P, Lobo SJM, Lobo S. Modeling and comparison of dissolution profiles. Eur J Pharm Sci 2001; 13(2): 123-33.
[http://dx.doi.org/10.1016/S0928-0987(01)00095-1] [PMID: 11297896]
[7]
Robinson JR, Lee VHL. Controlled Drug Delivery. (2nd ed.). New York: Marcel Dekker Inc 1987; p. 2930.
[8]
Pena R. Analysis of different parameters of an optimized prolonged release formulation obtained by five processes. Pharmaceutical Technology –Controlled Drug Release. London: Ellis Horwood 2005; pp. 57-70.
[9]
Mohanraj VJ, Chen Y. Nanoparticles – A review. Trop J Pharm Res 2007; 5(1): 561-73.
[10]
Kumaresh SS, Aminabhavi TM, Anandrao R. Biodegradable polymeric nanoparticles as drug delivery devices. J Control Release 2001; 70(1–2): 1-20.
[11]
Koohpar KZ, Entezari M, Movafagh A, Hashemi M. Anticancer activity of curcumin on human breast adenocarcinoma: Role of Mcl-1 gene. Iran J Cancer Prev 2015; 8(3): e2331.
[http://dx.doi.org/10.17795/ijcp2331] [PMID: 26413251]
[12]
Senthilraja P. in vitro cytotoxicity MTT assay in Vero, HepG2 and MCF -7 cell lines study of Marine. Yeast J Appl Pharmaceut Sci 2015; 5(03): 080-4.
[13]
Jayashree V. In - vitro anti-proliferative assay and cell viability activity of baicalein using breast cancer cell line. Int J Pharm Sci Res 2018; 9(4): 1620-4.
[14]
Mishra J, Dash AK, Kumar R. Nanotechnology challenges; nanomedicine: Nanorabots. Int Res J Pharm 2012; 2(4): 112-9.
[15]
Sailaja AK, Banu J, Sailaja AK, Banu J. Preparation and evaluation of chitosan loaded naproxen nanoparticles by emulsion interfacial reaction method. Drug Deliv Lett 2019; 9(2): 89-96.
[http://dx.doi.org/10.2174/2210303109666190211150117]
[16]
Sahoo SK, Panyam J, Prabha S, Labhasetwar V. Residual polyvinyl alcohol associated with poly (D,L-lactide-coglycolide) nanoparticles affects their physical properties and cellular uptake. J Control Release 2002; 82: 105-14.
[http://dx.doi.org/10.1016/S0168-3659(02)00127-X] [PMID: 12106981]
[17]
Sailaja AK, Swati P. Preparation and characterization of sulphasalazine loaded nanoparticles by nanoprecipitation and ionotropic gelation techniques using various polymers. Curr Nanomed 2017; 7(2): 125-41.
[18]
Patel B, Modi V, Komal PMP. Preparation and evaluation of ethyl cellulose microspheres prepared by emulsification - Solvent evaporation method. IJRMP 2012; 1(1): 2320-31.
[19]
Sailaja KA. Preparation of chitosan coated nanoparticles by emulsion polymerization technique. Asian J Pharm Clin Res 2011; 4(11): 73-4.
[20]
Sailaja AK, Amareshwar A, Chakravarty P. Different techniques used for the preparation of nanoparticles using natural polymers and their application. Int J Pharm Pharm Sci 2011; 3(2): 45-50.
[21]
Kreuter J. Nanoparticles. Colloidal Drug Delivery Systems. New York: Marcel Decker 1994; pp. 261-76.
[22]
Mueller RH. Colloidal Carriers for Controlled Drug Delivery and Targeting. Boston: CRC Press 1991; p. 379.
[23]
Krishna RSM. Nanoparticles: A novel colloidal drug delivery system. Indian J Pharma Edu Res 2006; 40(1): 15-21.
[24]
Le Thi MH, Chi NT, Triet NM, Le Ngoc TN, Chien DM. Preparation of drug nanoparticles by emulsion evaporation method Advanced Materials Science and Nanotechnology (AMSN08). J Phys Conf Ser 2009; 2009: 187.