Various technologies, like flow cytometry and cell sorting, have been established in fields of biomedical research. Fluorescence-activated cell sorting is one of the most powerful techniques to witness advancement in these years. This article aims to provide an in-depth overview of the FACS applications, along with regulatory considerations and qualification parameters for the instrument. Moreover, specifications of instruments from different brands with specialized features are mentioned. FACS is a cornerstone in clinical diagnostics. This review highlights the current advancements and versatility of this indispensable technology, and the said information would be a focal paradigm for the upcoming biomedical and pharmaceutical research.