Generic placeholder image

Cardiovascular & Hematological Disorders-Drug Targets

Author(s): Smail Amtaghri, Adil Qabouche, Miloudia Slaoui and Mohamed Eddouks*

DOI: 10.2174/011871529X255465231120055555

DownloadDownload PDF Flyer Cite As
Potential Antihypertensive Activity of the Aqueous Extract of Ammi visnaga and its Effect on ACE-2 in Rats

Page: [277 - 284] Pages: 8

  • * (Excluding Mailing and Handling)

Abstract

Aims: This work aimed to investigate the antihypertensive activity of Ammi visnaga. Background: The aqueous extract of Ammi visnaga has traditionally been used to treat hypertension in Morocco.

Objective: The objective of this investigation was to evaluate the effect of Ammi visnaga aqueous extract (AVAE) on arterial blood pressure, systolic blood pressure (SBP), mean blood pressure (MBP), diastolic blood pressure (DBP), and heart rate (HR) in normotensive and hypertensive rats. In addition, the effect of the aqueous extract of Ammi visnaga on vasodilatation was assessed in isolated rat aortic rings with functional endothelium pre-contracted with epinephrine EP or KCl.

Methods: AVAE was obtained, and its antihypertensive ability was pharmacologically investigated in L-NAME hypertensive and normotensive rats. The rats received oral AVAE at two selected doses of 70 and 140 mg/kg for six hours (acute experiment) and seven days (sub-chronic). Thereafter, systolic, diastolic, mean arterial blood pressure and heart rate were evaluated. Moreover, the vasorelaxant activity of AESA was performed in thoracic aortic ring rats. In addition, the mechanisms of action involved in the vasorelaxant effect were studied.

Results: AVAE lowered blood pressure only in L-Name-induced hypertensive rats. Furthermore, AVAE (0.375-1.375 mg/ml) showed a vasodilator effect in isolated aortic rats. In addition, not all of the medications used in our study were responsible for the signaling pathway. As a result, additional pharmaceuticals are required to confirm the mechanism of this signaling pathway.

Conclusion: The aqueous extract of Ammi visnaga exerts an interesting antihypertensive activity, which could be mediated through its vasorelaxant activity. The study supports its use as a medicinal plant against hypertension in Morocco.

Graphical Abstract

[1]
Raven, P.B.; Chapleau, M.W. Blood pressure regulation XI: Overview and future research directions. Eur. J. Appl. Physiol., 2014, 114(3), 579-586.
[http://dx.doi.org/10.1007/s00421-014-2823-z] [PMID: 24463603]
[2]
Oparil, S.; Acelajado, M.C.; Bakris, G.L.; Berlowitz, D.R.; Cífková, R.; Dominiczak, A.F.; Grassi, G.; Jordan, J.; Poulter, N.R.; Rodgers, A.; Whelton, P.K. Hypertension. Nat. Rev. Dis. Primers, 2018, 4(1), 18014.
[http://dx.doi.org/10.1038/nrdp.2018.14] [PMID: 29565029]
[3]
Zhou, B.; Perel, P.; Mensah, G.A.; Ezzati, M. Global epidemiology, health burden and effective interventions for elevated blood pressure and hypertension. Nat. Rev. Cardiol., 2021, 18(11), 785-802.
[http://dx.doi.org/10.1038/s41569-021-00559-8] [PMID: 34050340]
[4]
Es-Safi, I.; Mechchate, H.; Amaghnouje, A.; El Moussaoui, A.; Cerruti, P.; Avella, M.; Grafov, A.; Bousta, D. Marketing and legal status of phytomedicines and food supplements in Morocco. J. Complement. Integr. Med., 2021, 18(2), 279-285.
[http://dx.doi.org/10.1515/jcim-2020-0168] [PMID: 32950964]
[5]
Bellakhdar, J.; Claisse, R.; Fleurentin, J.; Younos, C. Repertory of standard herbal drugs in the Moroccan pharmacopoea. J. Ethnopharmacol., 1991, 35(2), 123-143.
[http://dx.doi.org/10.1016/0378-8741(91)90064-K] [PMID: 1809818]
[6]
Ziyyat, A.; Legssyer, A.; Mekhfi, H.; Dassouli, A.; Serhrouchni, M.; Benjelloun, W. Phytotherapy of hypertension and diabetes in oriental Morocco. J. Ethnopharmacol., 1997, 58(1), 45-54.
[http://dx.doi.org/10.1016/S0378-8741(97)00077-9] [PMID: 9324004]
[7]
Al-Douri, N.A.; Al-Essa, L.Y. A survey of plants used in Iraqi traditional medicine. Jordan J. Pharm. Sci., 2010, 3(2), 100-108.
[8]
Beltagy, A.M.; Beltagy, D.M. Chemical composition of Ammi visnaga L. and new cytotoxic activity of its constituents Khellin and Visnagin. Pharmac. Sci. Res., 2015, 7(6), 285.
[9]
Olufunke, M.D.; Kasali, A.A.; Olusegun, E. Constituents of the Spondias mombin Linn and the Comparison between its Fruit and Leaf essential oils. J. Essent. Oil-Bear. Plants, 2003, 6(3), 148-152.
[http://dx.doi.org/10.1080/0972-060X.2003.10643343]
[10]
Eddouks, M.; Maghrani, M.; Lemhadri, A.; Ouahidi, M.L.; Jouad, H. Ethnopharmacological survey of medicinal plants used for the treatment of diabetes mellitus, hypertension and cardiac diseases in the south-east region of Morocco (Tafilalet). J. Ethnopharmacol., 2002, 82(2-3), 97-103.
[http://dx.doi.org/10.1016/S0378-8741(02)00164-2] [PMID: 12241983]
[11]
Bellakhdar, J. Pharmacopée marocaine traditionnelle; Ibis press, 1997.
[12]
Meyer, U. From khellin to sodium cromoglycate--a tribute to the work of Dr. R. E. C. Altounyan (1922-1987). Pharmazie, 2002, 57(1), 62-69.
[PMID: 11836935]
[13]
Jouad, H.; Maghrani, M.; Eddouks, M. Hypoglycemic effect of aqueous extract of Ammi visnaga in normal and streptozotocin-induced diabetic rats. J. Herb. Pharmacother., 2002, 2(4), 19-29.
[http://dx.doi.org/10.1080/J157v02n04_03] [PMID: 15277079]
[14]
Hudson, J.; Towers, G.N. Phytomedicines as antivirals. Drugs Future, 1999, 24(3), 295.
[http://dx.doi.org/10.1358/dof.1999.024.03.858620]
[15]
Bencheraiet, R.; Kherrab, H.; Kabouche, A.; Kabouche, Z.; Maurice, J. Flavonols and antioxidant activity of Ammi visnaga L.(Apiaceae). Rec. Nat. Prod., 2011, 5(1), 52.
[16]
Pakfetrat, H.; Nemati, N.; Shiravi, A. Cytotoxicity effects of ammi visnaga extract on hela and mcf7 cancer cell line. J.Animal. Bio., 2015, 7(2), 25-33.
[17]
Kwon, M.S.; Lee, J.K.; Park, S.H.; Sim, Y.B.; Jung, J.S.; Won, M.H.; Kim, S.M.; Suh, H.W. Neuroprotective effect of visnagin on kainic acid-induced neuronal cell death in the mice hippocampus. Korean J. Physiol. Pharmacol., 2010, 14(5), 257-263.
[http://dx.doi.org/10.4196/kjpp.2010.14.5.257] [PMID: 21165322]
[18]
Lee, J.K.; Jung, J.S.; Park, S.H.; Park, S.H.; Sim, Y.B.; Kim, S.M.; Ha, T.S.; Suh, H.W. Anti-inflammatory effect of visnagin in lipopolysaccharide-stimulated BV-2 microglial cells. Arch. Pharm. Res., 2010, 33(11), 1843-1850.
[http://dx.doi.org/10.1007/s12272-010-1117-1] [PMID: 21116788]
[19]
Al-Snafi, A.E. A review of medicinal plants with broncho-dilatory effect-Part 1. Schol. Acad. J. Pharm., 2016, 5(7), 297-304.
[http://dx.doi.org/10.21276/sajp.2016.5.7.6]
[20]
Schimmer, O.; Rauch, P. Inhibition of metabolic activation of the promutagens, benzo[ a]pyrene, 2-aminofluorene and 2-aminoanthracene by furanochromones hi Salmonella typhimurium. Mutagenesis, 1998, 13(4), 385-389.
[http://dx.doi.org/10.1093/mutage/13.4.385] [PMID: 9717176]
[21]
Daoudi, A.; Aarab, L.; Abdel-Sattar, E. Screening of immunomodulatory activity of total and protein extracts of some Moroccan medicinal plants. Toxicol. Ind. Health, 2013, 29(3), 245-253.
[http://dx.doi.org/10.1177/0748233711430972] [PMID: 22301818]
[22]
Zgórka, G.; Dragan, T.; Głowniak, K.; Basiura, E. Determination of furanochromones and pyranocoumarins in drugs and Ammi visnaga fruits by combined solid-phase extraction–high-performance liquid chromatography and thin-layer chromatography-high-performance liquid chromatography. J. Chromatogr. A, 1998, 797(1-2), 305-309.
[http://dx.doi.org/10.1016/S0021-9673(97)01173-4] [PMID: 9542122]
[23]
Winderl, B.; Schwaiger, S.; Ganzera, M. Fast and improved separation of major coumarins in Ammi visnaga (L.) Lam. by supercritical fluid chromatography. J. Sep. Sci., 2016, 39(20), 4042-4048.
[http://dx.doi.org/10.1002/jssc.201600734] [PMID: 27514847]
[24]
Abu-Hashem, A.A.; El-Shazly, M. Synthesis, reactions and biological activities of furochromones: A review. Eur J Med Chem,, 2014, 90, 633-665.
[25]
Ajebli, M.; Eddouks, M. Antihypertensive activity of Petroselinum crispum through inhibition of vascular calcium channels in rats. J. Ethnopharmacol., 2019, 242, 112039.
[http://dx.doi.org/10.1016/j.jep.2019.112039] [PMID: 31252093]
[26]
Razzaq, M.A.; Younis, W.; Malik, M.N.H.; Alsahli, T.G.; Alamgeer; Jahan, S.; Ehsan, R.; Gasparotto Junior, A.; Bashir, A. Pulegone prevents hypertension through activation of muscarinic receptors and cyclooxygenase pathway in L-NAME-induced hypertensive rats. Cardiovasc. Ther., 2023, 2023, 1-17.
[http://dx.doi.org/10.1155/2023/8166840] [PMID: 37214130]
[27]
Calabrese, E.J.; Agathokleous, E.; Dhawan, G.; Kapoor, R.; Dhawan, V.; Manes, P.K.; Calabrese, V. Nitric oxide and hormesis. Nitric Oxide, 2023, 133, 1-17.
[http://dx.doi.org/10.1016/j.niox.2023.02.001] [PMID: 36764605]
[28]
Kiriyama, A.; Honbo, A.; Nishimura, A.; Shibata, N.; Iga, K. Pharmacokinetic-pharmacodynamic analyses of antihypertensive drugs, nifedipine and propranolol, in spontaneously hypertensive rats to investigate characteristics of effect and side effects. Regulatory Toxicology and Pharmacology, 2016, 76, 21-29.
[29]
Amthaghri, S.; Amssayef, A.; Slaoui, M.; Eddouks, M. Antihypertensive and vasorelaxant effects of hibiscus rosa-sinensis through angiotensin-converting enzyme-2 (ACE-2), and Ca2+ channels pathways. Cardiovascular & Hematological Disorders Drug Targets, 2022.