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Current Analytical Chemistry

Author(s): Jinqiu Xu, Yinhua Gong, Rongrong Huang, Liangliang Cai* and Xiaofei Ma*

DOI: 10.2174/0115734110270003231101115858

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Study on Separation Mechanism of Ephedrine and Pseudoephedrine in Borax Buffer by Capillary Electrophoresis

Page: [685 - 692] Pages: 8

  • * (Excluding Mailing and Handling)

Abstract

Background: In borax buffer, ephedrine (EPH) and pseudoephedrine (PSE) can be well separated (Rs>10) in capillary electrophoresis (CE) without adding any other compounds, but the specific mechanism remains unclear.

Methods: Titration analysis, nuclear magnetic resonance analysis, mass spectrum analysis and molecular modeling were used to investigate the separation mechanism.

Results: Tetrahydroxy borate (a hydrolyzate of borax or boric acid) could react with ephedrine and pseudoephedrine to form self-assembled complexes, but the two complexes had different stabilities and electrophoretic mobilities resulting from the different steric configurations of analytes.

Conclusion: The complexation based on borax was responsible for the separation.

Graphical Abstract

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