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Current Chinese Science

Author(s): Dong-min Cao* and Tao Liu

DOI: 10.2174/2210298103666230816090702

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Identification of Active Compounds of Pueraria Lobata and their Mechanisms of Action by LC-MS/MS and Network Pharmacology

Page: [398 - 406] Pages: 9

  • * (Excluding Mailing and Handling)

Abstract

Background: Osteoporosis has become a global public health problem, and Pueraria Lobata (PL) is a potential drug for treating osteoporosis.

Methods: Firstly, the components of PL were detected and identified based on UHPLC-Q-Exactive Orbitrap MS. In addition, we used network pharmacology to study the potential mechanism of PL in treating osteoporosis.

Result: A total of 48 compounds, including 38 isoflavones, 6 puerosides, and 4 others, were identified by UHPLC-Q Exactive-Orbitrap MS. The network of the pharmacological analysis revealed that 28 compounds of PL regulated 19 pathways through 27 targets, including estrogen signaling pathway and NF-kappa B signaling pathway, etc.

Conclusion: This study used LC-MS combined with network pharmacology to identify the compounds of PL and their mechanism of intervention in osteoporosis, which provides a scientific basis for PL to become a functional food for preventing osteoporosis.

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