Phytochemical Screening and Antioxidant Activity of Roots of Plumbago zeylanica L.

Article ID: e150823219724 Pages: 8

  • * (Excluding Mailing and Handling)

Abstract

Background: P. zeylanica L. is a well-known herbal plant that has long been used for its therapeutic properties. The present study aimed to carry out phytochemical screening to determine the total phenolic and flavonoid content as well as to evaluate the antioxidant properties of the methanolic extract of the root of Plumbago zeylanica L.

Methods: The antioxidant activity of the extract was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide scavenging, and hydroxyl radical scavenging assays.

Results: The phenolic and flavonoid contents of the extracts were 2.4% and 16.5%, respectively. P. zeylanica was found to scavenge free radicals with substantial antioxidant properties, exhibiting a high flavonoid and phenolic content. These results also suggest a strong correlation between antioxidant activities, as well as phenolic and flavonoid contents.

Conclusion: The plant sample exhibited superior free radical scavenging activity and phytochemical constituents that might be useful for further research on fighting oxidative stress.

[1]
Roy, A.; Bharadvaja, N. A review on pharmaceutically important medical plant: Plumbago zeylanica. Journal of Ayurvedic and Herbal Medicine, 2017, 3(4), 225-228.
[http://dx.doi.org/10.31254/jahm.2017.3411]
[2]
Rajakrishnan, R.; Lekshmi, R.; Benil, P.B.; Thomas, J.; AlFarhan, A.H.; Rakesh, V.; Khalaf, S. Phytochemical evaluation of roots of Plumbago zeylanica L. and assessment of its potential as a nephroprotective agent. Saudi J. Biol. Sci., 2017, 24(4), 760-766.
[http://dx.doi.org/10.1016/j.sjbs.2017.01.001] [PMID: 28490944]
[3]
Chauhan, M. A review on morphology, phytochemistry and pharmacological activities of medicinal herb Plumbago zeylanica Linn. Int J Pharmacog Phytochem Res., 2014, 3(2), 95-118.
[4]
Shukla, B; Saxena, S; Usmani, S; Kushwaha, P Phytochemistry and pharmacological studies of Plumbago zeylanica L. a medicinal plant review. Clinical Phytosci, 2021, 7(34)
[5]
Aleem, M. Anti-inflammatory and antimicrobial potential of Plumbago zeylanica L: a review. J. Drug Deliv. Ther., 2020, 10(5-s), 229-235.
[http://dx.doi.org/10.22270/jddt.v10i5-s.4445]
[6]
Gabriel, O.; Ademuyiwa, O.; Lasisi, A.A.; Olagunju, J.A. Free radical scavenging activities of extracts and bioactive constituents from the roots of Plumbago zeylanica (Linn.). Eur J Biol Med Sci Res., 2019, 7(2), 21-33.
[7]
Bloch, K.; Parihar, V.S.; Kellomäki, M.; Ghosh, S. Natural Compounds from Plumbago zeylanica as Complementary and Alternative Medicine. In: Chakraborti, S. (eds) In: Handbook of Oxidative Stress in Cancer: Therapeutic Aspects; Springer: Singapore, 2022.
[http://dx.doi.org/10.1007/978-981-16-1247-3_33-1]
[8]
Sahoo, S.; Ghosh, G.; Das, D.; Nayak, S. Phytochemical investigation and In vitro antioxidant activity of an indigenous medicinal plant Alpinia nigra B.L. Burtt. Asian Pac. J. Trop. Biomed., 2013, 3(11), 871-876.
[http://dx.doi.org/10.1016/S2221-1691(13)60171-9]
[9]
Kamali, M.; Khosroyar, S.; Kamali, H.; Ahmadzadeh Sani, T.; Mohammadi, A. Phytochemical screening and evaluation of antioxidant activities of Dracocephalum kotschyi and determination of its luteolin content. Avicenna J. Phytomed., 2016, 6(4), 425-433.
[PMID: 27516983]
[10]
Government of India Ministry of Health and Family Welfare Department of Ayush The Ayurvedic Pharmacopoeia of India, 2009, 4, 140-160.
[11]
Khandelwal, K.R.; Sethi, V. Practical Pharmacognosy; Pune; Nirali Prakashan, 2016, pp. 2,1-3,5.
[12]
Jijhotiya, A.; Goyal, S. Qualitative and quantitative phytochemical estimation of leaves extracts of plant Plumbago zeylanica. Int. J. Recent Sci. Res., 2018, 9, 23249-23252.
[13]
Hanani, E.; Soewandi, S.H.W.; Hayati H, H.; Revita, N. Pharmacognostical and preliminary phytochemical evaluation of cordia sebestena L. Pharmacogn. J., 2019, 11(5), 1100-1105.
[http://dx.doi.org/10.5530/pj.2019.11.172]
[14]
Jeevitha, M.; Ravi, P.V.; Subramaniyam, V.; Pichumani, M.; Sripathi, S.K. Exploring the phyto- and physicochemical evaluation, fluorescence characteristics, and antioxidant activities of Acacia ferruginea Dc: An endangered medicinal plant. Future J. Pharm. Sci., 2021, 7(1), 228.
[http://dx.doi.org/10.1186/s43094-021-00375-4]
[15]
Yu, M.; Gouvinhas, I.; Rocha, J.; Barros, A.I.R.N.A. Phytochemical and antioxidant analysis of medicinal and food plants towards bioactive food and pharmaceutical resources. Sci. Rep., 2021, 11(1), 10041.
[http://dx.doi.org/10.1038/s41598-021-89437-4] [PMID: 33976317]
[16]
Beyene, B.B.; Alem, F.A.; Ayana, M.T. Determination of antioxidant and antibacterial activities of leaf extracts of Plumbago zeylanica (Amira). Cogent Chem., 2020, 6(1), 1831715.
[http://dx.doi.org/10.1080/23312009.2020.1831715]
[17]
Agidew, M.G. Phytochemical analysis of some selected traditional medicinal plants in ethiopia. Bull. Natl. Res. Cent., 2022, 46(1), 87.
[http://dx.doi.org/10.1186/s42269-022-00770-8]