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Current Drug Therapy

Author(s): Neelam Singh, Puneet Gupta*, Radha Goel, Chaitanya Vinayak Narayan, Deepak Sagar and Aanchal Gupta

DOI: 10.2174/1574885518666230526124031

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Accelerating Healing of Excisional Wound with a Hydrogel Containing Hyacinth Bean (Dolichos lablab) Extract in a Rat Model

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Abstract

Introduction: Medicinal plants are essential in the treatment of a wide range of illnesses. Dolichos lablab, also known as Hyacinth Bean, is a popular legume crop in India that is rich in natural bioactive compounds. In recognition of its importance in many skin therapies, a hydrogel was prepared. The primary goal of topical formulations is to provide drug contact with the skin while reducing overall absorption. The aim of this study was to evaluate the healing effect of Dolichos lablab extract hydrogel on full-thickness wounds in rats.

Method: Wounds were created on the dorsal surface of Male Albino Wistar rats. Next, the animals were divided into four groups (n = 12). Each group was treated with 200 mg of the allotted topical treatment of blank gel, Dolichos hydrogel and marketed betadine gel. The treatments were applied immediately after the injury and twice daily for up to 14 days. Animals were euthanized on day 14 and some parameters were analyzed such as wound closure rate, Inflammatory cytokines level, oxidative stress profile, collagen hydroxyproline and hexosamine concentration, macroscopic and histopathological evidence.

Results: The findings indicate that Dolichos lablab extract has potent wound healing ability, as evidenced by improved wound closure and tissue regeneration, as well as histopathological parameters. Dolichos hydrogel increased the skin's hydroxyproline level, antioxidant potential, wound contraction, and anti-inflammatory activity by modulating the production of the cytokines TNF-α, IL-1β, and IL-6. This affirms the potency of the wound-healing properties of Dolichos lablab extract.

Conclusion: Dolichos lablab hydrogel is a low-cost herbal medicine which can aid in tissue repair.

Keywords: Dolichos lablab, hydrogel, excisional wound, cytokines, hydroxyproline, rats.

Graphical Abstract

[1]
Global burden of disease study. 2019. Available from: https://ghdx.healthdata.org/gbd-2019
[2]
Rajoo A, Ramanathan S, Mansor SM, Sasidharan S. Formulation and evaluation of wound healing activity of Elaeis guineensis Jacq leaves in a Staphylococcus aureus infected Sprague Dawley rat model. J Ethnopharmacol 2021; 266: 113414.
[http://dx.doi.org/10.1016/j.jep.2020.113414] [PMID: 32980488]
[3]
Rhea L, Dunnwald M. Murine excisional wound healing model and histological morphometric wound analysis. J Vis Exp 2020; 2020(162)
[4]
Venturella G, Ferraro V, Cirlincione F, Gargano ML. Medicinal mushrooms: Bioactive compounds, use, and clinical trials. Int J Mol Sci 2021; 22(2): 634.
[http://dx.doi.org/10.3390/ijms22020634] [PMID: 33435246]
[5]
Said dos Santos R, Bassi da Silva J, Rosseto HC, et al. Emulgels containing propolis and curcumin: The effect of type of vegetable oil, poly(acrylic acid) and bioactive agent on physicochemical stability, mechanical and rheological properties. Gels 2021; 7(3): 120.
[http://dx.doi.org/10.3390/gels7030120] [PMID: 34449614]
[6]
Molina GGV. Antimicrobial potentials of Dolichos lablab linn (Hyacinth Bean). Liceo J Higher Edu Res 2020; 16(2): 149-71.
[http://dx.doi.org/10.7828/ljher.v16i2.1382]
[7]
We are IntechOpen, the world ’ s leading publisher of Open Access books Built by scientists, for scientists TOP 1%. 2016. Available From: https://www.semanticscholar.org/paper/We-are-Intech Open-%2C-the-world-%E2%80%99-s-leading-publisher-Arribas-S%C3%A1nchez/b8213502e69a520f2c3a8b42af20b18971d1e99e
[8]
Habib HM, Theuri SW, Kheadr EE, Mohamed FE. Functional, bioactive, biochemical, and physicochemical properties of the Dolichos lablab bean. Food Funct 2017; 8(2): 872-80.
[http://dx.doi.org/10.1039/C6FO01162D] [PMID: 27918057]
[9]
Minde JJ, Venkataramana PB, Matemu AO. Dolichos Lablab-an underutilized crop with future potentials for food and nutrition security: A review. Crit Rev Food Sci Nutr Taylor & Franci 2021; 61: 2249-61.
[http://dx.doi.org/10.1080/10408398.2020.1775173]
[10]
Chen MX, Alexander KS, Baki G. Formulation and evaluation of antibacterial creams and gels containing metal ions for topical application. J Pharm 2016; 2016: 5754349.
[11]
Aggarwal G, Nagpal M. Pharmaceutical polymer gels in drug deliveryPolym Gels Perspect Appl. Singapore: Springer 2018; pp. 249-84.
[http://dx.doi.org/10.1007/978-981-10-6080-9_10]
[12]
Maslii Y, Ruban O, Kasparaviciene G, Kalveniene Z, et al. The influence of pH values on the rheological, textural and release properties of carbomer polacril® 40p-based dental gel formulation with plant-derived and synthetic active components. Molecules 2020; 25(21): 5018.
[13]
Balata G, El Nahas HM, Radwan S. Propolis organogel as a novel topical delivery system for treating wounds. Drug Deliv 2014; 21(1): 55-61.
[http://dx.doi.org/10.3109/10717544.2013.847032] [PMID: 24295500]
[14]
Rezano A, Indra T, Siregar Z, Maliki AS. Sappanwood (Caesalpinia sappan) extract gel do not heal skin excisional wound on Balb/c Mice. Biomed Pharmacol J 2019; 12: 375-81.
[15]
Nishinari K, Kohyama K, Kumagai H, Funami T, Bourne MC. Parameters of texture profile analysis. Food Sci Technol Res 2013; 19(3): 519-21.
[http://dx.doi.org/10.3136/fstr.19.519]
[16]
Yang GH, Lee YB, Kang D, et al. Overcome the barriers of the skin: Exosome therapy. Biomater Res 2021; 25(1): 22.
[http://dx.doi.org/10.1186/s40824-021-00224-8] [PMID: 34217362]
[17]
Zomer HD, Trentin AG. Skin wound healing in humans and mice: Challenges in translational research. J Dermatol Sci 2018; 90(1): 3-12.
[http://dx.doi.org/10.1016/j.jdermsci.2017.12.009] [PMID: 29289417]
[18]
Pourhoseingholi MA, Baghestani AR, Vahedi M. How to control confounding effects by statistical analysis. Gastroenterol Hepatol Bed Bench 2012; 5(2): 79-83.
[PMID: 24834204]
[19]
Howard B. Book review: Guide for the care and use of laboratory animals. Altern Lab Anim 1998; 26(3): 363-4.
[http://dx.doi.org/10.1177/026119299802600313]
[20]
Dev SK, Choudhury PK, Srivastava R, Sharma M. Antimicrobial, anti-inflammatory and wound healing activity of polyherbal formulation. Biomed Pharmacother 2019; 111: 555-67.
[http://dx.doi.org/10.1016/j.biopha.2018.12.075] [PMID: 30597309]
[21]
Reduan FH, Shaari RM, Sayuti NSA, et al. Acute and subacute dermal toxicity of ethanolic extract of Melastoma malabathricum leaves in Sprague-Dawley rats. Toxicol Res 2020; 36(3): 203-10.
[http://dx.doi.org/10.1007/s43188-019-00013-5] [PMID: 32685424]
[22]
Ali Khan B, Ullah S, Khan MK, Alshahrani SM, Braga VA. Formulation and evaluation of Ocimum basilicum-based emulgel for wound healing using animal model. Saudi Pharm J 2020; 28(12): 1842-50.
[http://dx.doi.org/10.1016/j.jsps.2020.11.011] [PMID: 33424273]
[23]
Eid BG, Alhakamy NA, Fahmy UA, et al. Melittin and diclofenac synergistically promote wound healing in a pathway involving TGF-β1. Pharmacol Res 2022; 175: 105993.
[http://dx.doi.org/10.1016/j.phrs.2021.105993] [PMID: 34801680]
[24]
Lim CY, In J. Randomization in clinical studies. Korean J Anesthesiol 2019; 72(3): 221-32.
[http://dx.doi.org/10.4097/kja.19049] [PMID: 30929415]
[25]
Kazemi M, Mohammadifar M, Aghadavoud E, Vakili Z, Aarabi MH, Talaei SA. Deep skin wound healing potential of lavender essential oil and licorice extract in a nanoemulsion form: Biochemical, histopathological and gene expression evidences. J Tissue Viability 2020; 29(2): 116-24.
[http://dx.doi.org/10.1016/j.jtv.2020.03.004] [PMID: 32204968]
[26]
Koshak AE, Algandaby MM, Mujallid MI, et al. Wound healing activity of Opuntia ficus-indica fixed oil formulated in a self-nanoemulsifying formulation. Int J Nanomedicine 2021; 16: 3889-905.
[http://dx.doi.org/10.2147/IJN.S299696] [PMID: 34135583]
[27]
Foltynski P, Ciechanowska A, Ladyzynski P. Wound surface area measurement methods. Biocybern Biomed Eng 2021; 41(4): 1454-65.
[http://dx.doi.org/10.1016/j.bbe.2021.04.011]
[28]
Khémiri I, Essghaier Hédi B, Sadfi Zouaoui N, Ben Gdara N, Bitri L. The antimicrobial and wound healing potential of Opuntia ficus indica L. inermis extracted oil from tunisia. Evid Based Complement Alternat Med 2019; 2019: 9148782.
[29]
Koriem KMM, Shamsuri RB, Ubaidillah AM. Evaluation of sodium fluoride toxicity in Schistosoma infected snails: Assessment of antioxidants, antiapoptotic, hypoprotein and hypocholesterol activities. J Parasit Dis 2016; 40(4): 1451-8.
[http://dx.doi.org/10.1007/s12639-015-0711-z] [PMID: 27876966]
[30]
Masson-Meyers DS, Andrade TAM, Caetano GF, et al. Experimental models and methods for cutaneous wound healing assessment. Int J Exp Pathol 2020; 101(1-2): 21-37.
[http://dx.doi.org/10.1111/iep.12346] [PMID: 32227524]
[31]
Dwivedi D, Dwivedi M, Malviya S, Singh V. Evaluation of wound healing, anti-microbial and antioxidant potential of Pongamia pinnata in wistar rats. J Tradit Complement Med 2016; 7(1): 79-85.
[32]
Libby P. Targeting inflammatory pathways in cardiovascular disease: The Inflammasome, interleukin-1, interleukin-6 and beyond. Cells 2021; 10(4): 951.
[http://dx.doi.org/10.3390/cells10040951] [PMID: 33924019]
[33]
Pereira LM, Hatanaka E, Martins EF, et al. Effect of oleic and linoleic acids on the inflammatory phase of wound healing in rats. Cell Biochem Funct 2008; 26(2): 197-204.
[http://dx.doi.org/10.1002/cbf.1432] [PMID: 17918246]
[34]
Grellner W, Georg T, Wilske J. Quantitative analysis of proinflammatory cytokines (IL-1β IL-6, TNF-α) in human skin wounds. Forensic Sci Int 2000; 113(1-3): 251-64.
[http://dx.doi.org/10.1016/S0379-0738(00)00218-8] [PMID: 10978634]
[35]
Pereira Beserra F, Sérgio Gushiken LF, Vieira AJ, et al. From inflammation to cutaneous repair: Topical application of lupeol improves skin wound healing in rats by modulating the cytokine levels, NF-κB, Ki-67, growth factor expression, and distribution of collagen fibers. Int J Mol Sci 2020; 21(14): 4952.
[http://dx.doi.org/10.3390/ijms21144952]
[36]
Bhavikatti SK, Karobari MI, Zainuddin SLA, et al. Investigating the antioxidant and cytocompatibility of Mimusops elengi Linn Extract over human gingival fibroblast cells. Int J Environ Res Public Health 2021; 18(13): 7162.
[http://dx.doi.org/10.3390/ijerph18137162] [PMID: 34281099]
[37]
Alsarayreh AZ, Oran SA, Shakhanbeh JM. Effect of Rhus coriaria L. methanolic fruit extract on wound healing in diabetic and non‐diabetic rats. J Cosmet Dermatol 2022; 21(8): 3567-77.
[http://dx.doi.org/10.1111/jocd.14668] [PMID: 34928525]
[38]
Ahmad SU, Binti Aladdin NA, Jamal JA, Shuid AN, Mohamed IN. Evaluation of wound-healing and antioxidant effects of marantodes pumilum (Blume) Kuntze in an excision wound model. 2021; 26(1): 228.
[39]
Maheshu V, Priyadarsini DT, Sasikumar JM. Effects of processing conditions on the stability of polyphenolic contents and antioxidant capacity of Dolichos lablab L. J Food Sci Technol 2013; 50(4): 731-8.
[http://dx.doi.org/10.1007/s13197-011-0387-z] [PMID: 24425975]
[40]
Zhang K, Zhang Y, Sun J, Meng J, Tao J. Deterioration of orthodox seeds during ageing: Influencing factors, physiological alterations and the role of reactive oxygen species. Plant Physiol Biochem 2021; 158: 475-85.
[http://dx.doi.org/10.1016/j.plaphy.2020.11.031] [PMID: 33250322]
[41]
Habib HM, Theuri SW, Kheadr E, Mohamed FE. DNA and BSA damage inhibitory activities, and anti-acetylcholinesterase, anti-porcine α-amylase and antioxidant properties of Dolichos lablab beans. Food Funct 2017; 8(2): 881-7.
[http://dx.doi.org/10.1039/C6FO01164K] [PMID: 27921109]
[42]
Ibrahim NI, Mohamed IN, Mohamed N, Mohd Ramli ES, Shuid AN. The effects of aqueous extract of Labisia Pumila (Blume) Fern.-Vill. Var. Alata on wound contraction, hydroxyproline content and histological assessments in superficial partial thickness of second-degree burn model. Front Pharmacol 2022; 13: 968664.
[http://dx.doi.org/10.3389/fphar.2022.968664] [PMID: 36313379]
[43]
Mathew-Steiner SS, Roy S, Sen CK. Collagen in wound healing. Bioengineering 2021; 8(5): 63.
[http://dx.doi.org/10.3390/bioengineering8050063] [PMID: 34064689]
[44]
Gunasekaran S, Nayagam AAJ, Natarajan R. Wound healing potentials of herbal ointment containing Calendula officinalis Linn. on the alteration of immunological markers and biochemical parameters in excision wounded animals. Clinical Phytoscience 2020; 6(1): 77.
[http://dx.doi.org/10.1186/s40816-020-00215-7]
[45]
Azis HA, Taher M, Ahmed AS, et al. In vitro and In vivo wound healing studies of methanolic fraction of Centella asiatica extract. S Afr J Bot 2017; 108: 163-74.
[http://dx.doi.org/10.1016/j.sajb.2016.10.022]
[46]
Banerjee K, Madhyastha R, Nakajima Y, Maruyama M, Madhyastha H. Nanoceutical adjuvants as wound healing material: Precepts and prospects. Int J Mol Sci 2021; 22(9): 4748.
[http://dx.doi.org/10.3390/ijms22094748] [PMID: 33947121]
[47]
Wynn TA, Vannella KM. Macrophages in tissue repair, regeneration, and fibrosis. Immunity 2016; 44(3): 450-62.
[http://dx.doi.org/10.1016/j.immuni.2016.02.015] [PMID: 26982353]
[48]
Nair A, Jacob S. A simple practice guide for dose conversion between animals and human. J Basic Clin Pharm 2016; 7(2): 27-31.
[http://dx.doi.org/10.4103/0976-0105.177703] [PMID: 27057123]
[49]
Tan JL, Lash B, Karami R, et al. Restoration of the healing microenvironment in diabetic wounds with matrix-binding IL-1 receptor antagonist. Commun Biol 2021; 4(1): 11.
[50]
Mirza RE, Fang MM, Ennis WJ, Koh TJ. Blocking interleukin-1β induces a healing-associated wound macrophage phenotype and improves healing in type 2 diabetes. Diabetes 2013; 62(7): 2579-87.
[http://dx.doi.org/10.2337/db12-1450] [PMID: 23493576]
[51]
Shukla SK, Sharma AK, Gupta V, Yashavarddhan MH. Pharmacological control of inflammation in wound healing. J Tissue Viability 2019; 28(4): 218-22.
[http://dx.doi.org/10.1016/j.jtv.2019.09.002] [PMID: 31542301]
[52]
Furuyama A, Hosokawa T, Mochitate K. Interleukin-1β and tumor necrosis factor-α have opposite effects on fibroblasts and epithelial cells during basement membrane formation. Matrix Biol 2008; 27(5): 429-40.
[http://dx.doi.org/10.1016/j.matbio.2008.02.005] [PMID: 18434122]
[53]
Dai J, Shen J, Chai Y, Chen H. IL-1 β impaired diabetic wound healing by regulating MMP-2 and MMP-9 through the p38 pathway. Mediators Inflamm 2021; 2021: 6645766.
[54]
Wu JR, Lu YC, Hung SJ, et al. Antimicrobial and immunomodulatory activity of herb extracts used in burn wound healing: “San Huang Powder”. Evid Based Complement Alternat Med 2021; 2021: 13.
[55]
Rolim LA, dos Santos FCM, Chaves LL, et al. Enhanced reader. Nature 2020; 2020: 539-47.
[56]
Ang L, Darwis Y, Koh R, et al. Wound healing property of curcuminoids as a microcapsule-incorporated cream. Pharmaceutics 2019; 11(5): 205.
[http://dx.doi.org/10.3390/pharmaceutics11050205] [PMID: 31052413]
[57]
Breijyeh Z, Jubeh B, Karaman R. Resistance of gram-negative bacteria to current antibacterial agents and approaches to resolve it. Molecules 2020; 25(6): 1340.
[http://dx.doi.org/10.3390/molecules25061340] [PMID: 32187986]
[58]
Barchitta M, Maugeri A, Favara G, et al. Nutrition and wound healing: An overview focusing on the beneficial effects of curcumin. Int J Mol Sci 2019; 20(5): 1119.
[http://dx.doi.org/10.3390/ijms20051119] [PMID: 30841550]