Abstract

A novel series of coumarin-benzimidazole hybrids were designed, synthesized, and extensively characterized using spectral data, including FT-IR, ¹H-NMR, and ¹³C-NMR. Weatherburn's method was used to test new compounds' inhibition of urease activity. The results were evaluated by considering the effects of different side groups and hybridization. The chlorine atom in the molecules notably affects the inhibitory activity of urease. The best result was obtained with the compound “6- chloro-N'-{[5,6-dichloro-2-(2-thienylmethyl)-1H-benzimidazol-1-yl]acetyl}-2-oxo-2H-chromene-3- carbohydrazide” (3b) with IC₅₀= 12.48±0.12 μg/mL against Jack bean urease.

Keywords: Hybridization, coumarin-benzimidazole hybrids, chlorine atom, urease activity, weatherburn's method, fused hybrids.