Abstract

The limited proteolysis by enteropeptidase of the recombinant chimeric proteins incorporating the linker (Asp)4- Lys was studied. The resistant to the hydrolysis chimeric proteins was shown to be the competitive inhibitors in re­ lation to the effectively splitting chimeric proteins and to the low molecular intermediates of the linker, but not of the trypsinogen processing. The linker was shown to be defined the binding of the protein substrates by enzyme only, rather than the efficiency, determined by the C-terminal component of enzyme specificity.